Oxygenated Form of Protocatechuate 3,4-Dioxygenase, a Non-heme Iron-containing Dioxygenase, as Reaction Intermediate
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Oxygenated form of protocatechuate 3,4-dioxygenase, a non-heme iron-containing dioxygenase, as reaction intermediate.
A short-lived new spectral species of protocatechuate 3,4-dioxygenase, a trivalent non-heme iron-containing enzyme, was observed in the early stage of the reaction. This new spectral species was characterized by a broad absorption band with a maximum between 500 and 520 rnp, distinct from those of the enzyme or the enzyme-protocatechuic acid complex. It could be demonstrated only in the presenc...
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In order to clarify the reaction mechanism of Mryptophan 2,3-dioxygenase (L-tryptophan: oxygen oxidoreductase, EC 1.13.1.12), a hemoprotein, spectral and kinetic studies were carried out with highly puritied enzyme preparations from Pseudomonas fluorescens (ATCC 11299). A new spectrally distinct species of the enzyme heme omx; 418,545, and 580 mp) was observed during the steady state of the cat...
متن کامل3 , 4 = Dioxygenase III . AN OXYGENATED FORM OF ENZYME AS REACTION INTERMEDIATE
In the early stage of the reaction of protocatechuate 3,4dioxygenase, a new spectral species of the enzyme having a broad absorption band with a maximum between 500 and 520 nm was observed by using a stopped flow technique. The observed spectrum was distinct from those of the enzyme or the enzyme-protoacatechuic acid complex and its formation was absolutely dependent on the simultaneous presenc...
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We have observed that high SOD-like function (decomposition of superoxide anion) was observed for several iron(III) compounds with tripodal ligands and several oxovanadium(IV) compounds, and also that these compounds exhibit high catalytic activity for oxidative cleavage of 3,5-di-tert-butylcatechol in non-donating solvents such as dichloromethane or nitromethane. These are suggesting that the ...
متن کاملThe reaction of oxygen with protocatechuate 3,4-dioxygenase from Pseudomonas putida. Characterization of a new oxygenated intermediate.
The reactions of protocatechuate dioxygenase (protocatechuate:oxygen 3,4-oxidoreductase, EC 1.13.11.3) with substrates and oxygen have been studied at 4 degrees C using rapid kinetic techniques. In this study, two oxygenated intermediates were kinetically and spectrally characterized. The rate of oxygen addition to the enzyme-substrate complex was determined to be 5 X 10(5) M-1 s-1. This oxygen...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)77223-8